PINS combines partitioning of single molecules into picoliter droplets, isothermal amplification, detection, and physical sorting in an automated microfluidic system.
In the first step of enrichment, the sample is diluted and distributed into millions of emulsion droplets. The DNA in all the droplets is amplified using the isothermal amplification enzyme Phi29, the presence of the target is detected in the individually amplified droplets by binding a fluorescent molecule specifically to the target DNA, and positive droplets are physically separated from negative droplets. The result of the procedure is an enrichment of long DNA fragments comprising the sequence used for detection.
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