Our next webinar – Certainty after CRISPR

April 20, 2021
15:00–16:30 CEST / 9:00–10:30 EDT

Precise genetic editing technologies allow researchers to excise, insert and alter targeted regions in genomes, opening new possibilities from gene therapies to plant breeding. Coupled to that potential is an effort to make edits tractable and certain. Join Keyi Geng and Eric Paul Bennett in this two-part webinar discussing unanticipated alterations happening after gene editing and ways to detect and characterize expected and unexpected outcomes.

Where did the target region go? Unravelling odd CRISPR/Cas9-induced genomic alterations in human cells

Keyi Geng, PhD Student, Karolinska Institute

  • Two cancer model cell lines were modfied with CRISPR-Cas9 to excise a region of interest, but show evidence that the targeted sequence is still present and functional in the modified cells

  • Target-specific PCR and Sanger sequencing, however, appeared to confirm the removal of the region of interest from the intended edit site

  • In-depth analysis revealed surprising events – including rearrangements and reinsertions – happening after CRISPR/Cas9-induced sequence excision
Keyi Geng

Keyi received her Master's Degree from Shanghai Jiao Tong University (China) working on cerebral ischemia and cellular senescence. She is now a PhD student in Claudia Kutter's lab at the Karolinska Institute (Stockholm, Sweden), focusing on deciphering tRNA gene functionality using CRISPR/Cas9 and various sequencing technologies.

Fast and sensitive detection of indels induced by precise gene targeting

Eric Paul Bennett, MSc, Dr. Med., COBO Technologies

  • Brief survey of the pros and cons of current gene editing InDel detection methodologies

  • Are InDels the only outcomes to be expected after gene editing?

  • Choice of “fit for purpose” InDel detection methodology

EBennett

Eric has decades of experience establishing precision engineered isogenic cells lacking individual genes involved in glycosylation. He has spearheaded methods and workflows to address unmet needs in the field, improving the targeting and indel detection efficacy of precise genome editing applications. He is a co-founder of COBO Technologies.

What you’ll learn from this webinar

  • Learn about potential unintended modifications during and after CRISPR/Cas9-induced edits
  • See how targeted long-read sequencing can reveal otherwise undetected genome alterations
  • Gain insights into available methods to validate the accuracy and precision of small and large genome edits
  • Get an overview of the advantages and disadvantages of different validation methods