April 20, 2021
15:00–16:30 CEST / 9:00–10:30 EDT
Precise genetic editing technologies allow researchers to excise, insert and alter targeted regions in genomes, opening new possibilities from gene therapies to plant breeding. Coupled to that potential is an effort to make edits tractable and certain. Join Keyi Geng and Eric Paul Bennett in this two-part webinar discussing unanticipated alterations happening after gene editing and ways to detect and characterize expected and unexpected outcomes.
Keyi Geng, PhD Student, Karolinska Institute
Two cancer model cell lines were modfied with CRISPR-Cas9 to excise a region of interest, but show evidence that the targeted sequence is still present and functional in the modified cells
Target-specific PCR and Sanger sequencing, however, appeared to confirm the removal of the region of interest from the intended edit site
Keyi received her Master's Degree from Shanghai Jiao Tong University (China) working on cerebral ischemia and cellular senescence. She is now a PhD student in Claudia Kutter's lab at the Karolinska Institute (Stockholm, Sweden), focusing on deciphering tRNA gene functionality using CRISPR/Cas9 and various sequencing technologies.
Eric Paul Bennett, MSc, Dr. Med., COBO Technologies
Brief survey of the pros and cons of current gene editing InDel detection methodologies
Are InDels the only outcomes to be expected after gene editing?
Choice of “fit for purpose” InDel detection methodology
Eric has decades of experience establishing precision engineered isogenic cells lacking individual genes involved in glycosylation. He has spearheaded methods and workflows to address unmet needs in the field, improving the targeting and indel detection efficacy of precise genome editing applications. He is a co-founder of COBO Technologies.
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