CRISPR Validation Services

How certain are you that your CRISPR edits worked? Look beyond the edit site. Xdrop™ reveals unintended modifications where other methods fail.

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Four Winners of Samplix Xdrop™ Grant Program have been awarded!

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CRISPR Validation Services Overview

Get in-depth validation of your CRISPR-engineered samples with Samplix.

  • Scrutinise > 40 kb around the edited site in depth (~100-fold coverage)
  • Detect unintended modifications e.g. loss of heterozygosity, rearrangements
  • Bypass limitations of standard PCR-based validations


Xdrop™ approach

Xdrop™ Indirect Sequence Capture will enrich for all long DNA fragments displaying a Detection Sequence placed 5-10 kb apart from the edited site. The enriched DNA is sequenced on both Oxford Nanopore and Illumina platforms, to get detailed information about both structural rearrangements and SNPs. 

You can check how we detected a plasmid insertion in different cell lines in our Application Note and BioRxiv paper.

 

design crispr services

 

Don't hesitate in contacting us. Fill the form below and learn more in details how we can help you validating your samples.

 

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Our customers say... 

“We used Samplix Xdrop™ and Sequencing Services in a project aiming at closing genomic gaps of the herring Y chromosome. We were pleased with the advice we received from Samplix experts regarding experimental design as well as with the speed and quality of the delivered Service”

Prof. Leif Andersson, Uppsala University, Sweden

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