droplet Multiple Displacement Amplification

Unbiased amplification of single long DNA sequences
containing the target.

Uniform sequence coverage.

No inter-template chimeras.

The key is that dMDA happens in droplets

mdma workflow

Partitioning one single DNA molecule in each droplet extremely reduces amplification bias:

  • no preferred amplification of certain templates
  • no nested products
  • no inter-template chimera


Amplifying every template in separate droplets (droplet MDA) provides a highly uniform sequence coverage, while bulk MDA preferentially amplifies certain templates.

And why MDA?

    • MDA amplifies any DNA, employing no specific primers (uses random hexamer primers)
    • The high-fidelity, proofreading Phi29 enzyme has a 10-fold lower error rate than Taq polymerase
    • The isothermal reaction leaves more template DNA intact
    • MDA preferentially amplifies sequences 1–70 kb long

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Hexamer primers bind at Multiple sites. Growing strands are Displaced by others, opening additional primer annealing sites to further boost Amplification.

Here’s how it works in detail

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Encapsulate Target DNA

Xdrop® generates thousands of droplets, each with single Target DNA + amplification reagents

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Unbiased Samplicon Production

Each molecule of Target DNA undergoes multiple displacement amplification in droplets

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Release of ~ 1µg of Target DNA

Break solution releases Samplicons (amplified Target DNA) into solution

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Downstream Analysis

Target is enriched 100-1000x and is ready for long- and short-read sequencing, qPCR etc.

Ready for applications....

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See the whole flow

Brochure workflow SAMPLIX A3

See how droplet MDA works

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Our VP explains ISC & dMDA

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