Unbiased amplification of single long DNA sequences
containing the target.
Uniform sequence coverage.
No inter-template chimeras.
Partitioning one single DNA molecule in each droplet extremely reduces amplification bias:
Amplifying every template in separate droplets (droplet MDA) provides a highly uniform sequence coverage, while bulk MDA preferentially amplifies certain templates.
Hexamer primers bind at Multiple sites. Growing strands are Displaced by others, opening additional primer annealing sites to further boost Amplification.
Xdrop® generates thousands of droplets, each with single Target DNA + amplification reagents
Each molecule of Target DNA undergoes multiple displacement amplification in droplets
Break solution releases Samplicons (amplified Target DNA) into solution
Target is enriched 100-1000x and is ready for long- and short-read sequencing, qPCR etc.
Ready for applications....
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