Overcoming challenges in plant genomics

Plant Biology Europe 2021
June 29, 2021 at 15:00 CEST

Xdrop: targeted enrichment to overcome challenges in long- and short-read sequencing of large and complex plant genomes

With more than 20 years of experience in the life sciences industry, Peter Mouritzen heads market and application development at Samplix. His team's efforts focus on expanding and optimizing known application and discovering new applications for the Xdrop® technology.

Leading into the conference session on "Genomics and Genome Editing for Crop Design", Peter will provide an overview of how Xdrop is being used to elucidate the complexity of plant genomes and validate editing for engineered crops.

Abstract

Xdrop is a microfluidic droplet technology that enriches and amplifies long DNA fragments of largely unknown sequence from low sample input. Genomics researchers have coupled it with long- and short-read sequencing (Oxford Nanopore, PacBio and Illumina) to gain high-resolution insights into genomic regions that have previously been inaccessible. Examples of its use in unraveling plant genomes include closing gaps due to poor genome assemblies or low correspondence between reference genome and plant variety, resolving structural rearrangements like transposable elements, and examining biosynthetic gene clusters without performing whole genome sequencing. Xdrop can be applied in relation to QTL mapping and bulk segregant analysis by enabling targeted sequencing of the identified region of interest in several segregating plants with the aim of identifying the polymorphisms either linked to or causing the phenotype of interest. This presentation introduces how the long-range genomic information (100 kb) and single-molecule resolution achieved with Xdrop target enrichment simplifies working with large and structurally complex genomes with an easy capture design and workflow.

Peter M

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