DNA workflow Capture and/or amplify long DNA fragment (>100kb)
Each step of the DNA workflow can be done independently depending on the application.
Indirect sequence capture (Xdrop®)
Amplicon based
Hybridization-capture
This protocol is a modification of the official Xdrop® DNA enrichment protocol. Relative to the targeted enrichment protocol, the procedure described here does not use droplet MDA to amplify the DNA isolated in the double-emulsion (DE) droplets. Instead of droplet MDA, long-range PCR is applied using primers specific to adapters ligated onto DNA ends of fragmented DNA. The fragmentation and adapter ligation are performed on the input DNA prior to DNA encapsulation in DE droplets.
Double-emulsion droplets can easily be sorted.
Sorting can be done with our new instrument Xdrop Sort or by flow cytometry.
Unbiased amplification of single long DNA sequences containing the target Uniform sequence coverage No inter-template chimeras
Partitioning one single DNA molecule in each droplet extremely reduces amplification bias:
Amplifying every template in separate droplets (droplet MDA) provides a highly uniform sequence coverage, while bulk MDA preferentially amplifies certain templates.
