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Technical notes

This section includes technical notes describing experiments that establish some of the capabilities of our Xdrop microfluidics system.

Featured: Duplex Xdrop enrichment

To better elucidate the steps of the Xdrop workflow and how these are connected, we enriched DNA to sequence a well-known gene and highlight the outcome expectations at various milestones from sample preparation to read mapping.

BRCA2 is a human tumor suppressor gene located on chromosome 13. The protein it codes for is responsible for DNA repair. BRCA2 is involved in preventing genomic rearrangements that can be oncogenic, and many of the mutations identified to date in this gene correlate with increased risk of cancer.

Xdrop technical notes

Samplix Xdrop TechNote Improved WGA Jan 2022

Improved WGA with Xdrop®

BRCA2 Milestones Tech note V1.0

Duplex Xdrop® enrichment

Samplix Xdrop TechNote Droplet compatibility with growth media Jan 2022

Droplet compatibility with media

2007 Samplix technote benchmarking v1.0

Benchmarking Xdrop®

Brochures

Xdrop Fact sheet v0 1

Xdrop® fact sheet

Brochure workflow SAMPLIX A3

Xdrop® workflow

Xdrop flyer

Xdrop® flyer

Posters

Poster Single molecule amplification

Unbiased Amplification of Single Molecules enables even coverage of chromosomal DNA

Videos

Learn more about the process and product in these videos. 

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Samplix Technology - Indirect Sequence Capture with Xdrop

Learn about how you can enrich for long DNA fragments on your region of interest using the Xdrop technology. Using a short Detection Sequence (around 150 bp) you can Indirectly Capture long fragments (more than 100 kb long), select and amplify them for sequencing on long or short read platforms.

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How does Xdrop work? A tour in the lab

Xdrop workflow encompasses two main processes: Indirect Sequence Capture using double emulsion droplets and Multiple displacement amplification in single emulsion droplets. You start with nanograms of high molecular weight DNA and end with micrograms of enriched long DNA fragments.

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Xdrop- Droplet PCR for Indirect Sequence Capture

The Xdrop instrument makes it easy to enrich long DNA fragments for targeted long- and short-read sequencing. The first stage of the Xdrop enrichment process involves partitioning DNA into millions of double emulsion droplets using the Xdrop instrument. These droplets are highly stable and are suitable for PCR cycling and flow cytometry sorting. This simple protocol makes it possible for any laboratory to start enriching for their DNA target of choice.

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Xdrop - Multiple Displacement Amplification in droplets (dMDA)

The Xdrop instrument from Samplix makes it easy unbiased amplification of single DNA molecules. Each molecule is partitioned into separated single emulsion droplets, where the amplification occurs.
Multiple displacement amplification in droplets compared to bulk, provides a much uniform coverage, no inter-template chimeras and no preferential template amplification.