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This section includes technical notes describing experiments that establish some of the capabilities of our Xdrop microfluidics system.
To better elucidate the steps of the Xdrop workflow and how these are connected, we enriched DNA to sequence a well-known gene and highlight the outcome expectations at various milestones from sample preparation to read mapping.
BRCA2 is a human tumor suppressor gene located on chromosome 13. The protein it codes for is responsible for DNA repair. BRCA2 is involved in preventing genomic rearrangements that can be oncogenic, and many of the mutations identified to date in this gene correlate with increased risk of cancer.
Improved WGA with Xdrop®
Duplex Xdrop® enrichment
Droplet compatibility with media
Xdrop® fact sheet
Unbiased Amplification of Single Molecules enables even coverage of chromosomal DNA
Learn about how you can enrich for long DNA fragments on your region of interest using the Xdrop technology. Using a short Detection Sequence (around 150 bp) you can Indirectly Capture long fragments (more than 100 kb long), select and amplify them for sequencing on long or short read platforms.
Xdrop workflow encompasses two main processes: Indirect Sequence Capture using double emulsion droplets and Multiple displacement amplification in single emulsion droplets. You start with nanograms of high molecular weight DNA and end with micrograms of enriched long DNA fragments.
The Xdrop instrument makes it easy to enrich long DNA fragments for targeted long- and short-read sequencing. The first stage of the Xdrop enrichment process involves partitioning DNA into millions of double emulsion droplets using the Xdrop instrument. These droplets are highly stable and are suitable for PCR cycling and flow cytometry sorting. This simple protocol makes it possible for any laboratory to start enriching for their DNA target of choice.
The Xdrop instrument from Samplix makes it easy unbiased amplification of single DNA molecules. Each molecule is partitioned into separated single emulsion droplets, where the amplification occurs.
Multiple displacement amplification in droplets compared to bulk, provides a much uniform coverage, no inter-template chimeras and no preferential template amplification.
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Samplix ApS is a biotechnology company. Our website includes information on our proprietary products and their applications in gene and cell screening, as well as our services offering. It's possible to create a customer account to use bioinformatics tools used for our workflows.