Targeted NGS

With Xdrop™ in your lab, your target enrichment becomes as easy to set up as a standard PCR analysis.

Take advantage of a streamlined setup and run multiple targets in parallel, or check your cloning status and DNA integration of any transgenes, CRISPR edited genes with Xdrop™

  • Quick and easy enrichment of any gene or genomic region by the Xdrop™ platform

  • Capture up to 100 kb of unknown sequence with one standard primer set

  • Quick and easy protocol – ideal for any custom target enrichment

  • Run 8 regions, or 8 samples per chip

  • Automated droplet generation in microfluidics chips

dna

With the Xdrop™ platform, any genomic region is equally well enriched. Even GC-rich and/or repetitive regions are easily captured with Xdrop™ making the system an ideal alternative to long-range PCR, custom probe-based target enrichment and whole genome sequencing solutions.

Even though long-range PCR is widely used, this method can be challenging especially when trying to uncover large regions (tens of kilobases). To fully unravel sequence information of large regions, multiple long-range PCR primer sets are needed, and each primer set needs to be independently optimized.

This process can both be laborious, time-consuming and difficult for large genomic regions, especially those which contain difficult-to-PCR regions.

Failures and long-range PCR primer re-designs make the process time consuming and the process requires extensive hands-on time. Furthermore, PCR product - and sample management are challenging, as well as repeated PCR product quantifications, normalizations, pooling and library preparation. Also, long-range PCR requires full sequence knowledge of the targeted region.

Other technologies for target enrichment are for example custom DNA or RNA probe based bait enrichment or PCR based library preparations.

These methods can however be difficult to apply to certain genomic regions, synthesis of baits or primer pools often cause long delivery times, are generally expensive, and have minimum sample number restrictions which may not fit with the project size. They also demand full sequence knowledge of the targeted region.

Xdrop™ platform from Samplix solves these problems with an easy-to-use process allowing NGS libraries to be constructed within a week from ordering a primer set for a given target region

Xdrop™ DNA amplification is PCR-free which reduces bias and errors typically found in PCR-based methods.

Only limited sequence information is needed for each region making Xdrop™ a perfect choice for de-novo gene region sequencing based on transcriptome data or target organisms where genome information is limited (e.g. plants).

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