Upcoming Webinar: Pitfalls and best practices for CRISPR/Cas9-based gene editing

May 25, 2021
14:00 GMT / 15:00 CEST / 9:00 EST

Experience with genome editing has revealed a number of pitfalls and missteps that can happen along the way. Join our experts in a compelling discussion about what you should know before tackling a genome editing project. They raise important questions, highlight what to be aware of, and provide best practices to ensure your edits worked as intended.

10 things you should think of when designing a good gene editing strategy

Benjamin Schmid, Research Scientist, Cellular Engineering and Disease Models, Bioneer A/S

Bioneer A/S has been working with different genome editing techniques for almost 10 years and has been through the evolution of gene editing. During the last 5 years, CRISPR/Cas9 has been the gene editing tool of choice. Dr. Benjamin Schmid will present lessons learnt and key quality control steps during a gene editing project. 

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How to ensure your gene editing validation accounts for larger unintended rearrangements

Dr. Peter Mouritzen, VP Application & Market Development, Samplix

Enabling precise genetic modifications, CRISPR-based editing techniques have redefined how we explore the genome and may allow engineering tomorrow’s medicines or better crops. Validating CRISPR edits, however, is not that straightforward. Commonly used methods have missed critical unintended alterations. Dr. Peter Mouritzen discusses an expanded quality control for unintended modifications by covering a larger region around the gene edit and demonstrates this with examples to establish a comprehensive validation strategy. 

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What you’ll learn from this webinar

  • Learn how to plan a good gene-editing strategy 
  • Don’t rush into it - learn about the most common pitfalls using CRISPR/Cas9 
  • Improved technologies and workflow to maximize success rate 
  • What needs to be checked after a successful gene edit?
  • See examples of unintended alterations from CRISPR editing that can go undetected 
  • Discover the importance of resolving edit patterns and rearrangements 
  • Learn how to enrich and sequence long DNA fragments to validate CRISPR edits 
  • Gain insights into the selection of an appropriate validation method