Use Xdrop to encapsulate mammalian or microbial cells in double-emulsion droplets for single-cell assays.
Xdrop is a versatile and user-friendly instrument for preparing living mammalian or microbial cells, organelles, DNA, or other biological material for high-resolution downstream analyses.
Using our Xdrop Cartridges, Xdrop safely and rapidly encapsulates the living cells, organelles, or DNA fragments together with assay chemistry in highly stable, picoliter-volume, double-emulsion droplets. These droplets are stable through pipetting, vortexing, incubation, flow cytometry, sorting, and even long-term storage. What's more, cells can be recovered from the droplets for expansion.
Together with appropriate analytics technologies, Xdrop applications include:
With Xdrop, researchers can more easily reach high-resolution insights into the cellular functionality and genomics of humans, animals, plants, and microorganisms.
Xdrop can also generate single-emulsion droplets for other workflows, including to encapsulate DNA for unbiased whole genome amplification.
We supply the complete range of reagents, cartridges, and accessories required for Xdrop. What’s more, user training is included as part of our standard Xdrop installation service.
See the full range of Xdrop Cartridges for encapsulating single mammalian cells, single microbial cells, organelles, and DNA.
These proprietary microfluidics cartridges are designed by Samplix for fully self-contained droplet generation on Xdrop and Xdrop Sort. Samplix also provides the full range of required reagents.
Our newest cartridge is designed to produce highly stable, ~100-picoliter, double-emulsion droplets to encapsulate mammalian cells, transforming bulk-cell into single-cell assays. Workflows using the Xdrop DE50 Cartridge include cytokine secretion and cell killing assays. Simply load the reagents and samples, put the cartridge in Xdrop, and run the program.
Natural killer cells encapsulated in DE50 droplets, which have a volume of ~100 pl
Produce highly stable, ~1.5-picoliter, double-emulsion droplets to encapsulate millions microbial cells, organelles, or DNA with the self-contained Xdrop DE20 Cartridge. Each droplet acts as a compartment for single-cell resolution assays or targeted enrichment of DNA. Simply load the reagents and samples, put the cartridge in Xdrop or Xdrop Sort, and run the program.
Sort double-emulsion droplets containing fluorescing DNA or small cells with the new Xdrop DE20 Sort Cartridge. Designed for use with our new instrument, Xdrop Sort, this user-friendly system requires no expertise with fluorescence-based sorters. Simply load the reagents and samples, put the cartridge in Xdrop Sort, and run the sorting program.
Produce single-emulsion droplets to encapsulate millions of molecules, organelles, or cells with the self-contained Xdrop SE85 Cartridge. Each droplet acts as a compartment for single-molecule or single-cell resolution assays. Simply load the reagents and samples, put the cartridge in Xdrop or Xdrop Sort, and run the program.
Both instruments run targeted DNA enrichment workflows to prepare genomic material from large libraries for screening. They can both also encapsulate living cells for a range of cell-based applications.
Xdrop Sort uniquely enables DNA or cell encapsulation and high-throughput sorting. This enables the screening of large, complex gene libraries with unprecedented speed.
This new member of the Xdrop family can both generate and sort double-emulsion droplets in a user-friendly workflow that does not require any experience with fluorescence-based cell sorters.
The workflow starts with capture of 100 kb DNA fragments, small single cells, or other biological material in highly stable droplets for assays like PCR and enzyme activity assessment. Then, based on content fluorescence, droplets are sorted to obtain only the content of interest for high-resolution downstream analyses such as:
Xdrop generates two sizes of double-emulsion droplets: DE20 and DE50. The Xdrop DE20 Cartridge generates DE20 droplets, which are ~20 µm in diameter (volume: ~1.6 picoliters) and are suitable for microbial cell workflows. The Xdrop DE50 Cartridge generates DE50 droplets, which are ~50 µm in diameter (volume: ~100 picoliters) and are suitable for mammalian cell workflows. Xdrop Sort generates DE20 droplets and can also sort them based on fluorescence signals.
