Accessible to every lab, Xdrop microfluidics products are changing how we look at cells.
Get the most precise view of the function and potency of members of your immune cell population. Quickly encapsulate millions of single living cells in microfluidics droplets that are compatible with flow cytometers, cell sorters, and incubators to get the insights you need for immune therapy research.
Xdrop single-cell cytotoxicity assay, combined with Bio-Rad StarBright™ Dye-conjugated fluorescent antibodies, enables precise profiling of CAR T cells. It facilitates quantification of cytotoxic CD8+ CAR T cells and reveals a distinct cytotoxic CD4+ CAR T cells. Unlike bulk assays, this single-cell assay in droplets enables in-depth functional characterization of CAR T cells and CAR cassette construct designs.
Co-encapsulation of immune and cancer cells enables high-throughput functional analysis and droplet sorting based on parameters such as cytotoxicity, caspase activation, granzyme B or cytokine release. This is followed by molecular profiling, including RNA sequencing. Further investigation aims to uncover its full potential.
With single-cell assays in droplets, Xdrop reveals the heterogeneity of CAR T cells. In this study we showed that only about 20% kill their targets within 6 hours, while 33% secrete active granzyme B. Such detailed insights into cellular heterogeneity are not achievable with traditional bulk assays.
This droplet-based flow cytometry workflow enables simultaneous detection of granzyme B secretion and cell killing with single-cell resolution. It uses Xdrop DE50 droplets to encapsulate natural killer cells together with their targets for incubation and flow cytometry. Our findings here support the widely accepted dynamics of granzyme B secretion and cell killing, showing the utility of Xdrop in advancing our understanding of vital cell biology processes and as a powerful tool for immune cell therapy development.
In this collaboration with Agilent Technologies, we looked at the potential to rapidly identify active NK cells in a cell population. We used our Xdrop droplet-based format for an assay on the Agilent NovoCyte Quanteon flow cytometer. Target cell killing was seen within an hour of incubation, allowing the identification of the fastest killers. The assay is fast as the picoliter droplet volume leads to rapid cell–cell interactions, marking a significant advancement in the study and analysis of immune cells.
Bulk functional assays of immune cells miss highly potent cells within the population. This Xdrop workflow reveals highly potent TNF-α-secreting T cells in a human blood sample.
View our published papers.
Transform discovery workflows. Effortlessly encapsulate single-cell libraries, enhancing the speed of your screening campaigns.
Accelerate discovery with high-throughput screening. Encapsulate millions of antibody-secreting cells to uncover rare, high-value variants.
Advance genomics workflows with precise single DNA fragment encapsulation. Ideal for insert validation and targeted enrichments.
