Accessible to every lab, Xdrop microfluidics products are changing how we look at cells.
Get the most precise view of the function and potency of members of your immune cell population. Quickly encapsulate millions of single living cells in microfluidics droplets that are compatible with flow cytometers, cell sorters, and incubators to get the insights you need for immune therapy research.
With single-cell assays in droplets, Xdrop reveals the heterogeneity of CAR T cells. In this study we showed that only about 20% kill their targets within 6 hours, while 33% secrete active granzyme B. Such detailed insights into cellular heterogeneity are not achievable with traditional bulk assays.
This droplet-based flow cytometry workflow enables simultaneous detection of granzyme B secretion and cell killing with single-cell resolution. It uses Xdrop DE50 droplets to encapsulate natural killer cells together with their targets for incubation and flow cytometry. Our findings here support the widely accepted dynamics of granzyme B secretion and cell killing, showing the utility of Xdrop in advancing our understanding of vital cell biology processes and as a powerful tool for immune cell therapy development.
In this collaboration with Agilent Technologies, we looked at the potential to rapidly identify active NK cells in a cell population. We used our Xdrop droplet-based format for an assay on the Agilent NovoCyte Quanteon flow cytometer. Target cell killing was seen within an hour of incubation, allowing the identification of the fastest killers. The assay is fast as the picoliter droplet volume leads to rapid cell–cell interactions, marking a significant advancement in the study and analysis of immune cells.
Bulk functional assays of immune cells miss highly potent cells within the population. This Xdrop single-cell format workflow for IFN-γ secretion assessment reveals these highly potent individual cells, enabling their retrieval and expansion.
Quantifying individual cells that secrete IFN-γ, TNF-α, or both cytokines in a single-cell, Xdrop®-based workflow.
This Xdrop workflow enables rapid, multiplex quantification of the individual immune cells in a population that are secreting one or more cytokines. The results for single-cell format multiplex and singleplex assay concurred and were reproducible.
Bulk functional assays of immune cells miss highly potent cells within the population. This Xdrop workflow reveals highly potent TNF-α-secreting T cells in a human blood sample.
Starting from just 10 ng of DNA, Xdrop reveals gene cassettes inserted using lentivirus and other transduction systems. Here, Xdrop reveals 1,000 unique insertion sites of the CAR cassette inserted all over the genome.
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Identifying functional subpopulations of IFN-y, TNF-a, GzmB secretors and actively killing effector cells to understand heterogeneity in adoptive cell therapy
Xdrop: Revealing functional heterogeneity of effector cells for enhanced adoptive cell therapy applications
Identifying and isolating single immune cells based on their function using an Xdrop workflow
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2024
Analyzing functional heterogeneity of effector cells for enhanced adoptive cell therapy applications
Anne-Christine Kiel Rasmussen, Thomas Morgan Hulen, David Leander Petersen, Mette Juul Jacobsen, Marie Just Mikkelsen, Özcan Met, Marco Donia, Christopher Aled Chamberlain, Peter Mouritzen
Immuno-Oncology and Technology, Volume 24, 100738 (2024) DOI: https://doi.org/10.1016/j.iotech.2024.100738
2023
Long-read whole genome analysis of human single cells
Hård, J., Mold, J.E., Eisfeldt, J. et al.
Nat Commun 14, 5164 (2023). DOI: https://doi.org/10.1038/s41467-023-40898-3
2020
Corrigendum to "Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line" [Stem Cell Res. 34/1873-5061 (2019) 101349-55]
Schmid B, Prehn KR, Nimsanor N, Garcia BIA, Poulsen U, Jørring I, Rasmussen MA, Clausen C, Mau-Holzmann UA, Ramakrishna S, Muddashetty R, Steeg R, Bruce K, Mackintosh P, Ebneth A, Holst B, Cabrera-Socorro A.
Stem Cell Res. 2020 Sep 21;48:102005. doi: 10.1016/j.scr.2020.102005. Epub ahead of print. Erratum for: Stem Cell Res. 2019 Jan;34:101349. PMID: 32971461.
Transform discovery workflows. Effortlessly encapsulate single-cell libraries, enhancing the speed of your screening campaigns.
Elevate cell line development workflow. Encapsulate and screen single mammalian cells for enhanced antibody production.
Advance genomics workflows with precise single DNA fragment encapsulation. Ideal for insert validation and targeted enrichments.
