Investigate the true function
and potency of immune cells

Accessible to every lab, Xdrop facilitates single-cell cytokine,
granzyme, antibody, and cell killing assays.

Use Xdrop for immunotherapy research

Get the most precise view of the function and potency of members of your immune cell population. Quickly encapsulate millions of single living cells in microfluidics droplets that are compatible with flow cytometers, cell sorters, and incubators to get the insights you need for immune therapy research.

Application notes

Revealing granzyme B secretion and cell killing dynamics in a single-cell format

This droplet-based flow cytometry workflow enables simultaneous detection of granzyme B secretion and cell killing with single-cell resolution. It uses Xdrop DE50 droplets to encapsulate natural killer cells together with their targets for incubation and flow cytometry. Our findings here support the widely accepted dynamics of granzyme B secretion and cell killing, showing the utility of Xdrop in advancing our understanding of vital cell biology processes and as a powerful tool for immune cell therapy development.

Application notes

Rapidly identifying active natural killer cells

In this collaboration with Agilent Technologies, we looked at the potential to rapidly identify active NK cells in a cell population. We used our Xdrop droplet-based format for an assay on the Agilent NovoCyte Quanteon flow cytometer. Target cell killing was seen within an hour of incubation, allowing the identification of the fastest killers. The assay is fast as the picoliter droplet volume leads to rapid cell–cell interactions, marking a significant advancement in the study and analysis of immune cells.

Cell killing

Application notes

Revealing and retrieving highly potent IFN-γ secretors using an Xdrop single-cell format workflow 

Bulk functional assays of immune cells miss highly potent cells within the population.
This Xdrop single-cell format workflow for IFN-γ secretion assessment reveals these highly potent individual cells, enabling their retrieval and expansion.

Single cell assay

Application notes

Single cell assay to quantify IFN-γ and TNF-α secretion

Quantifying individual cells that secrete IFN-γ, TNF-α, or both cytokines in a single-cell, Xdrop®-based workflow.

This Xdrop workflow enables rapid, multiplex quantification of the individual immune cells in a population that are secreting one or more cytokines.
The results for single-cell format multiplex and singleplex assay concurred and were reproducible.

Quantifying INF and TFN secretion

Application notes

Identifying highly potent TNF-α-secreting T cells from blood samples in 7 hours using the Xdrop® double-emulsion droplet-based workflow.

Bulk functional assays of immune cells miss highly potent cells within the population.
This Xdrop workflow reveals highly potent TNF-α-secreting T cells in a human blood sample.

TNF-a secretors

Application notes

Localizing lentiviral transduction-inserted CAR cassettes in T cells using Xdrop®

Starting from just 10 ng of DNA, Xdrop reveals gene cassettes inserted using lentivirus and other transduction systems.
Here, Xdrop reveals 1,000 unique insertion sites of the CAR cassette inserted all over the genome.

Insertion site density

Posters

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Poster identifying and isolating single immune cells based on their function

Identifying and isolating single immune cells based on their function using an Xdrop workflow

Scientific papers

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2023

Long-read whole genome analysis of human single cells

Hård, J., Mold, J.E., Eisfeldt, J. et al.

Nat Commun 14, 5164 (2023). DOI: https://doi.org/10.1038/s41467-023-40898-3

2020 

Corrigendum to "Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line" [Stem Cell Res. 34/1873-5061 (2019) 101349-55]

Schmid B, Prehn KR, Nimsanor N, Garcia BIA, Poulsen U, Jørring I, Rasmussen MA, Clausen C, Mau-Holzmann UA, Ramakrishna S, Muddashetty R, Steeg R, Bruce K, Mackintosh P, Ebneth A, Holst B, Cabrera-Socorro A.

Stem Cell Res. 2020 Sep 21;48:102005. doi: 10.1016/j.scr.2020.102005. Epub ahead of print. Erratum for: Stem Cell Res. 2019 Jan;34:101349. PMID: 32971461.

Discover other applications

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Screening

Transform discovery workflows. Effortlessly encapsulate single-cell libraries, enhancing the speed of your screening campaigns.


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Cell line development

Elevate cell line development workflow. Encapsulate and screen single mammalian cells for enhanced antibody production.


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Genomics

Advance genomics workflows with precise single DNA fragment encapsulation. Ideal for insert validation and targeted enrichments.