Whether you're screening mammalian or microbial cells Xdrop microfluidics products can boost your speed and accuracy.
Improve the throughput and efficiency of mammalian and microbial cell screening. Encapsulate millions of living cells in microfluidics droplets that are compatible with flow cytometers and cell sorters, meaning you can screen and retrieve the cells with the desired properties for further analysis.
Improving the throughput and efficiency of screening for rare, active enzyme variants in very large libraries.
During bulk cultivation, bacterial cells producing inactive enzyme variants can outgrow cells producing active but rare variants, making screening ineffective.
Xdrop greatly increases the throughput for fluorescence-based screening, improving enzyme engineering efficiency.
With single-cell assays in droplets, Xdrop reveals the heterogeneity of CAR T cells. In this study we showed that only about 20% kill their targets within 6 hours, while 33% secrete active granzyme B. Such detailed insights into cellular heterogeneity are not achievable with traditional bulk assays.
This droplet-based flow cytometry workflow enables simultaneous detection of granzyme B secretion and cell killing with single-cell resolution. It uses Xdrop DE50 droplets to encapsulate natural killer cells together with their targets for incubation and flow cytometry. Our findings here support the widely accepted dynamics of granzyme B secretion and cell killing, showing the utility of Xdrop in advancing our understanding of vital cell biology processes and as a powerful tool for immune cell therapy development.
Revealing and retrieving highly potent IFN-γ secretors using an Xdrop® single-cell format workflow based on double-emulsion droplets.
Bulk functional assays of immune cells miss highly potent cells within the population.
This Xdrop single-cell format workflow for IFN-γ secretion assessment reveals these highly potent individual cells, enabling their retrieval and expansion.
This Xdrop workflow enables rapid, multiplex quantificationof the individual immune cells ina population that are secreting one or more cytokines.
The results for single-cell format multiplex and singleplex assay concurred and were reproducible.
Identifying highly potent TNF-α-secreting T cells from blood samples in 7 hours using the Xdrop® double-emulsion droplet-based workflow.
This Xdrop workflow reveals highly potent TNF-α-secreting T cells in a human blood sample.
Encapsulating individual living yeast cells for screening and recovery with Xdrop® Sort.
Living yeast cells can be encapsulated in Xdrop DE20 (1.6 pl) or DE50 (100 pl) droplets.
The yeast cells can proliferate in DE50 droplets.
Based on their fluorescence, DE20 droplets containing yeast cells can be sorted using Xdrop Sort in a workflow that is sensitive, accurate, and high-throughput.
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Identifying and isolating single immune cells based on their function using an Xdrop workflow
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Explore immune cell function in single-cell format assays, including cytokine, granzyme B, and cell killing assays.
Elevate cell line development workflow. Encapsulate and screen single mammalian cells for enhanced antibody production.
Advance genomics workflows with precise single DNA fragment encapsulation. Ideal for insert validation and targeted enrichments.
