Manuals, guides & tools

Find useful information about Samplix products, including manuals, quick guides, workflow recommendations, instructional videos, and safety data sheets.

Digital tools

We provide a suite of digital tools to our customers to support droplet sorting and sequencing workflows. The sequencing-related tools include calculators to predict enrichment, primer designers for the detection and validation sequences, and software for downstream tasks, such as base calling, reference downloads, and generating reports. Learn more on our dedicated tools page.

 EES0816(4)

Manuals, quick guides, and protocols

Manuals

The instrument manuals describes in details all the steps and materials required to produce or sort double emulsion droplets. 

 

Quick guides

The quick guides summarize the steps for producing and breaking the various droplet types in a handy single-page document.

For information on producing DE50 droplets and using the Xdrop Well Insert, please contact us.

Recommendations and user-developed protocols

The recommendations and user-developed protocols (UDPs) give details for workflows and partial workflows developed by Xdrop users at Samplix or partner labs. These workflows may involve instruments that are not developed by Samplix (e.g., DNA library sequencers, flow cytometers). Some adaptation and validation may be required.

Droplets for manuals page

Xdrop fact sheets

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Fact sheet on using Xdrop for cell-focused workflows

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Fact sheet on Xdrop DE and SE droplets

Xdrop Fact Sheet

Xdrop fact sheet

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Xdrop Sort fact sheet

2007 Samplix technote benchmarking v1.0

Benchmarking Xdrop

Videos

Learn more about the process and product in these videos. 

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Samplix Technology - Indirect Sequence Capture with Xdrop

Learn about how you can enrich for long DNA fragments on your region of interest using the Xdrop technology. Using a short Detection Sequence (around 150 bp) you can Indirectly Capture long fragments (more than 100 kb long), select and amplify them for sequencing on long or short read platforms.

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How does Xdrop work? A tour in the lab

Xdrop workflow encompasses two main processes: Indirect Sequence Capture using double emulsion droplets and Multiple displacement amplification in single emulsion droplets. You start with nanograms of high molecular weight DNA and end with micrograms of enriched long DNA fragments.

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Xdrop- Droplet PCR for Indirect Sequence Capture

The Xdrop instrument makes it easy to enrich long DNA fragments for targeted long- and short-read sequencing. The first stage of the Xdrop enrichment process involves partitioning DNA into millions of double emulsion droplets using the Xdrop instrument. These droplets are highly stable and are suitable for PCR cycling and flow cytometry sorting. This simple protocol makes it possible for any laboratory to start enriching for their DNA target of choice.

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Xdrop - Multiple Displacement Amplification in droplets (dMDA)

The Xdrop instrument from Samplix makes it easy unbiased amplification of single DNA molecules. Each molecule is partitioned into separated single emulsion droplets, where the amplification occurs.
Multiple displacement amplification in droplets compared to bulk, provides a much uniform coverage, no inter-template chimeras and no preferential template amplification.