Find useful information about Samplix products, including manuals, quick guides, workflow recommendations, instructional videos, and safety data sheets.
We provide a suite of digital tools to our customers to support droplet sorting and sequencing workflows. The sequencing-related tools include calculators to predict enrichment, primer designers for the detection and validation sequences, and software for downstream tasks, such as base calling, reference downloads, and generating reports. Learn more on our dedicated tools page.
Contains workflows for producing droplets with Xdrop DE20, Xdrop DE50, or Xdrop SE85 Cartridges to contain living microbial cells, living mammalian cells, fragments of DNA, or other biological material.
Contains workflows for producing droplets with Xdrop DE20 or Xdrop SE85 Cartridges to contain living microbial cells, fragments of DNA, or other biological material; and for sorting droplets containing such material with Xdrop DE20 Sort Cartridges.
Addendum: The Xdrop Sort Idle function has been added to the software. This guide describes how to use this function.
The quick guides summarize the steps for producing and breaking the various droplet types in a handy single-page document.
The recommendations and user-developed protocols (UDPs) give details for workflows and partial workflows developed by Xdrop users at Samplix or partner labs. These workflows may involve instruments that are not developed by Samplix (e.g., DNA library sequencers, flow cytometers). Some adaptation and validation may be required.
Fact sheet on using Xdrop for cell-focused workflows
Fact sheet on Xdrop DE and SE droplets
Xdrop fact sheet
Xdrop Sort fact sheet
Learn about how you can enrich for long DNA fragments on your region of interest using the Xdrop technology. Using a short Detection Sequence (around 150 bp) you can Indirectly Capture long fragments (more than 100 kb long), select and amplify them for sequencing on long or short read platforms.
Xdrop workflow encompasses two main processes: Indirect Sequence Capture using double emulsion droplets and Multiple displacement amplification in single emulsion droplets. You start with nanograms of high molecular weight DNA and end with micrograms of enriched long DNA fragments.
The Xdrop instrument makes it easy to enrich long DNA fragments for targeted long- and short-read sequencing. The first stage of the Xdrop enrichment process involves partitioning DNA into millions of double emulsion droplets using the Xdrop instrument. These droplets are highly stable and are suitable for PCR cycling and flow cytometry sorting. This simple protocol makes it possible for any laboratory to start enriching for their DNA target of choice.
The Xdrop instrument from Samplix makes it easy unbiased amplification of single DNA molecules. Each molecule is partitioned into separated single emulsion droplets, where the amplification occurs.
Multiple displacement amplification in droplets compared to bulk, provides a much uniform coverage, no inter-template chimeras and no preferential template amplification.
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Samplix ApS is a biotechnology company. Our website includes information on our proprietary products and their applications in gene and cell screening. It's possible to create a customer account to use bioinformatics tools used for our workflows.