This short video shows the straightforward steps to prepare an Xdrop DE20 Cartridge for droplet production. DE20 droplets are suitable for small cells (e.g., microbial cells) or genomic material (e.g., DNA fragments). This process requires an Xdrop or Xdrop Sort, an Xdrop DE20 Cartridge and Gasket, Xdrop DE Oil, an appropriate sample mix (e.g., cell sample and growth medium), outer phase (e.g., growth medium), and a pipette.
In this short video, we demonstrate how to collect DE20 droplets from an Xdrop DE20 Cartridge after a production run. The video includes instructions for how to prepare the droplets for droplet PCR (if the contents are DNA fragments). This process requires an Xdrop DE20 Cartridge with freshly produced DE20 droplets containing small cells or DNA fragments, a pipette, and 1.5 ml LoBind DNA tubes. For droplet PCR, you will also need PCR tubes.
This short video shows how to prepare an Xdrop DE50 Cartridge for droplet production. DE50 droplets are suitable for mammalian or microbial cells. This process requires an Xdrop or Xdrop Sort, an Xdrop DE50 Cartridge and Gasket, Xdrop DE Oil, an appropriate sample mix (e.g., cell sample and growth medium) and outer phase (e.g., growth medium), and a pipette.
When you have encapsulated cells in DE50 droplets, follow this straightforward procedure to collect them for incubation, analysis on a flow cytometer, or sorting on a cell sorter. The cells can stay alive in the droplets for several days. This process requires an Xdrop DE50 Cartridge with freshly produced DE50 droplets containing mammalian or microbial cells, a pipette, and 2.0 ml LoBind tubes.
Xdrop SE85 droplets are single-emulsion droplets used in some of our genomics workflows. This short video shows you how to prepare an Xdrop SE85 Cartridge for the droplet MDA workflow or single-cell whole genome amplification. This process requires an Xdrop or Xdrop Sort, Xdrop SE85 Cartridge, Holder, and Gasket, a pipette, Droplet Oil (SE), and the sample mix.
When you have encapsulated DNA in SE85 droplets, follow this straightforward pipetting procedure to transfer them to collection tubes. It's important to pipette SE85 droplets in the manner shown for the best collection results. The process requires an Xdrop SE85 Cartridge in an Xdrop SE85 Holder with freshly produced SE85 droplets containing DNA, a pipette, and collection tubes.
In just two minutes, learn how to stain the DNA inside DE20 droplets to facilitate sorting based on fluorescence. It's an important step in our droplet-based targeted enrichment workflow. This process requires DNA in DE20 droplets after droplet-based PCR, which can be done on a thermal cycler. It also requires a pipette, DE staining buffer, and a 1.5 ml LoBind DNA tube.
Xdrop DE20 droplets can be sorted based on the fluorescence of their contents. This three-minute video shows how to prepare an Xdrop DE20 Sort Cartridge prior to loading it into your Xdrop Sort. This process requires an Xdrop Sort, an Xdrop DE20 Sort Cartridge and Gasket, DE20 droplets containing stained cells or DNA, Xdrop foil for sorting, an Xdrop Sort Lane Opener, Xdrop Blank Oil Droplets, and DE sorting buffer.
This one-minute video shows how easy it is to set up and run the droplet sorting program on Xdrop Sort. After inserting the Xdrop DE20 Sort Cartridge with its precious load of DE20 droplets containing stained DNA or small cells, you use the intuitive touchscreen interface to customise a few settings. A standard sorting run takes just 40 minutes and requires an Xdrop Sort and an appropriately loaded Xdrop DE20 Sort Cartridge.
After a sorting run on Xdrop Sort, use this straightforward procedure to collect the droplets containing the DNA or small cells with the desired fluorescent signal. For this process, you need an Xdrop DE20 Sort Cartridge containing sorted droplets, a pipette, 1.5 ml collection tubes, and a bench top centrifuge.
See how straightforward it is to encapsulate your sample (e.g., cells or DNA fragments) in double-emulsion (DE) droplets. In the video, an Xdrop DE50 Cartridge is loaded with natural killer cells and lymphoblasts, which are then encapsulated for a cell-killing assay in single-cell format. Droplets will contain a natural killer cell, a lymphoblast, or both cell types; some will be empty. The droplets with a single cell or no cells act as controls.
See how straightforward it is to sort your sample (e.g., microbial cells or DNA fragments) when it is encapsulated in double-emulsion (DE) droplets. Pipette the droplets and media into the Xdrop DE20 Sort Cartridge and start your run on Xdrop Sort. In the video, an Xdrop DE20 Sort Cartridge is loaded with droplets containing DNA fragments. Sorting occurs based on fluorescence. You can adjust the detection threshold to ensure you capture all of your precious sample.
The Xdrop instrument makes it easy to enrich long DNA fragments for targeted long- and short-read sequencing. The first stage of the Xdrop enrichment process involves partitioning DNA into millions of double emulsion droplets using the Xdrop instrument. These droplets are highly stable and are suitable for PCR cycling and flow cytometry sorting. This simple protocol makes it possible for any laboratory to start enriching for their DNA target of choice.
The Xdrop instrument from Samplix makes it easy unbiased amplification of single DNA molecules. Each molecule is partitioned into separated single emulsion droplets, where the amplification occurs. Multiple displacement amplification in droplets (dMDA) compared to bulk, provides a much uniform coverage, no inter-template chimeras and no preferential template amplification.
