This short video shows the straightforward steps to prepare an Xdrop DE20 Cartridge for droplet production. DE20 droplets are suitable for small cells (e.g., microbial cells) or genomic material (e.g., DNA fragments). This process requires an Xdrop or Xdrop Sort, an Xdrop DE20 Cartridge and Gasket, Xdrop DE Oil, an appropriate sample mix (e.g., cell sample and growth medium), outer phase (e.g., growth medium), and a pipette.
In this short video, we demonstrate how to collect DE20 droplets from an Xdrop DE20 Cartridge after a production run. The video includes instructions for how to prepare the droplets for droplet PCR (if the contents are DNA fragments). This process requires an Xdrop DE20 Cartridge with freshly produced DE20 droplets containing small cells or DNA fragments, a pipette, and 1.5 ml LoBind DNA tubes. For droplet PCR, you will also need PCR tubes.
This short video shows how to prepare an Xdrop DE50 Cartridge for droplet production. DE50 droplets are suitable for mammalian or microbial cells. This process requires an Xdrop or Xdrop Sort, an Xdrop DE50 Cartridge and Gasket, Xdrop DE Oil, an appropriate sample mix (e.g., cell sample and growth medium) and outer phase (e.g., growth medium), and a pipette.
When you have encapsulated cells in DE50 droplets, follow this straightforward procedure to collect them for incubation, analysis on a flow cytometer, or sorting on a cell sorter. The cells can stay alive in the droplets for several days. This process requires an Xdrop DE50 Cartridge with freshly produced DE50 droplets containing mammalian or microbial cells, a pipette, and 2.0 ml LoBind tubes.
In just two minutes, learn how to stain the DNA inside DE20 droplets to facilitate sorting based on fluorescence. It's an important step in our droplet-based targeted enrichment workflow. This process requires DNA in DE20 droplets after droplet-based PCR, which can be done on a thermal cycler. It also requires a pipette, DE staining buffer, and a 1.5 ml LoBind DNA tube.
See how straightforward it is to encapsulate your sample (e.g., cells or DNA fragments) in double-emulsion (DE) droplets. In the video, an Xdrop DE50 Cartridge is loaded with natural killer cells and lymphoblasts, which are then encapsulated for a cell-killing assay in single-cell format. Droplets will contain a natural killer cell, a lymphoblast, or both cell types; some will be empty. The droplets with a single cell or no cells act as controls.
