Xdrop changes how you can analyze cells

See how our unique microfluidics platform boosts the speed and accuracy of
functional analyses of mammalian or microbial cells.

Create the ideal home for functional analyses of cells

Killer cell assay with staining white

Killer cell assay with staining white

What could you achieve if you boosted the speed and accuracy of your functional analyses of living cells? Find out with our proprietary benchtop instrument, Xdrop.

Xdrop encapsulates living cells in highly stable 
double-emulsion droplets that act as picoliter-sized microenvironments for incubation and downstream analyses.

Xdrop can change how you analyze cells by transforming bulk functional analyses into rapid and accurate assays with
single-cell resolution.

Pictured: co-encapsulated lymphoblasts and natural killer cells with staining to show successful immune reaction

How are double-emulsion droplets beneficial?

E coli expressing cellulase

Xdrop's double-emulsion droplets act as picoliter-sized reaction chambers or microenvironments for your cells, allowing you to:

  • Accelerate workflows by increasing the rate of cell–cell interactions or buildup of cell secretions
  • Gain a single-cell view that reveals functional heterogeneity
  • Vastly simplify workflows with straightforward protocols on our benchtop instrument
  • With appropriate sorting technology, recover the cells with the desired properties

 

What functional assays does Xdrop support?

Xdrop has proven performance in a range of single-cell resolution functional assays, including:

  • Cell killing by human immune cells
  • Cytokine secretion by mammalian cells
  • Enzyme secretion by microbial cells

This shows our unique instrument's potential for engineered cell therapy research, molecular engineering, synthetic biology, and microbial research. 

Pictured: Bright field (top) and merged bright field and fluorescent (bottom) images of cellulase-expressing E. coli cell in a double-emulsion droplet (provided by the Schwaneberg Group, RWTH Aachen University)

E coli expressing cellulase

Tiny chambers or microenvironments for your cells

 42P6149 edited

 42P6149 edited

Discover Xdrop DE20 and DE50 droplets

Xdrop generates two sizes of double-emulsion droplets. The Xdrop DE20 Cartridge generates the smaller droplets, which are ~20 µm in diameter (volume: ~1.5 picoliters) and are suitable for microbial cell workflows. The Xdrop DE50 Cartridge generates the larger size, which are ~50 µm in diameter (volume: ~100 picoliters) and are suitable for mammalian cell workflows.

Pictured: The channels on an Xdrop DE20 Cartridge in which the reagents mix to form ~20-µm diameter droplets

Xdrop DE50 Cartridge

Cells are happy in DE20 and DE50 droplets

Cells are encapsulated within the droplets in growth medium. Small molecules, such as carbon dioxide and water, can diffuse across the barrier. However, large, complex molecules, such as cytokines and enzymes, remain inside the droplet.

Pictured: The top and underside of an Xdrop DE50 Cartridge in which the reagents mix to form 50-µm diameter droplets

Xdrop DE50 Cartridge

A highly versatile instrument

Validating CRISPR edits and CAR-T cassette insertions is an important part of cell therapy research. Xdrop has protocols developed for long- and short-read sequencing of DNA to verify such edits. The workflow involves encapsulating DNA for targeted enrichment in double-emulsion droplets. Downstream sequencing reveals unintended on-target and off-target rearrangements that other methods cannot easily find.

What our customers say

  
“We have had a long collaboration with the Samplix team. They have listened to our feedback, and we are glad to see how far this technology has come since the early days when we received the world’s first Xdrop in our lab.”

- Dr. Adam Ameur, Uppsala University & SciLifeLab, Sweden

Dr. Adam Ameur, Uppsala University and SciLifeLab

Photo credit: Uppsala University

“We investigate a complex genomic and molecular interaction induced by CRISPR-Cas9 deletion. It requires highly efficient and sensitive target enrichment followed by long-read sequencing. The Samplix team is very knowledgeable and experienced in the specifics of our project. We also received very helpful advice on the data analysis.”

- Keyi Geng, Karolinska Institutet, Sweden

Keyi Geng, Karolinska Instituet, Sweden

“We worked with Samplix on a project aiming to close genomic gaps of the herring Y chromosome. We were pleased with the advice we received from Samplix experts regarding experimental design as well as with the speed and quality of the results.”

- Prof. Leif Andersson, Uppsala University, Sweden

Professor Leif Andersson, Uppsala University, Sweden

Photographer: Mikael Wallerstedt