Boost the speed and accuracy of functional analyses of cells

Use Xdrop to encapsulate mammalian or microbial cells in double-emulsion droplets for single-cell assays.

Xdrop®

Xdrop is a versatile and user-friendly instrument for preparing living mammalian or microbial cells, organelles, DNA, or other biological material for high-resolution downstream analyses.

Using our Xdrop Cartridges, Xdrop safely and rapidly encapsulates the living cells, organelles, or DNA fragments together with assay chemistry in highly stable, picoliter-volume, double-emulsion droplets. These droplets are stable through pipetting, vortexing, incubation, flow cytometry, sorting, and even long-term storage. What's more, cells can be recovered from the droplets for expansion.

Together with appropriate analytics technologies, Xdrop applications include:

  • Cell killing by human immune cells
  • Cytokine secretion by mammalian cells
  • Enzyme secretion by microbial cells
  • Verification of gene edits, including assessment of unintended
    on- and off-target rearrangments

With Xdrop, researchers can more easily reach high-resolution insights into the cellular functionality and genomics of humans, animals, plants, and microorganisms.

Xdrop can also generate single-emulsion droplets for other workflows, including to encapsulate DNA for unbiased whole genome amplification. 

We supply the complete range of reagents, cartridges, and accessories required for Xdrop. What’s more, user training is included as part of our standard Xdrop installation service.

Specifications

Width: 25 cm   9.8 inches
Height: 25 cm   9.8 inches
Length: 48 cm   18.9 inches
Weight: 17 kg   37.5 lbs
Voltage requirements:  110-240 V

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Why choose Xdrop?

  • Xdrop can encapsulate living mammalian or microbial cells in highly stable double-emulsion droplets for functional assays, incubation, phenotyping, and screening.
  • Xdrop helps answer key questions in engineered cell and gene therapy research.
  • Xdrop supports molecular engineering workflows, including enzyme evolution and pathway engineering.
  • Xdrop has a user-friendly interface and a small footprint to make the power of microfluidics accessible to every molecular biology laboratory.

Cartridges and reagents

See the full range of Xdrop Cartridges for encapsulating single mammalian cells, single microbial cells, organelles, and DNA.

These proprietary microfluidics cartridges are designed by Samplix for fully self-contained droplet generation on Xdrop and Xdrop Sort. Samplix also provides the full range of required reagents.

New: Xdrop® DE50 Cartridge

For encapsulating mammalian cells in double-emulsion droplets

Our newest cartridge is designed to produce highly stable, ~100-picoliter, double-emulsion droplets to encapsulate mammalian cells, transforming bulk-cell into single-cell assays. Workflows using the Xdrop DE50 Cartridge include cytokine secretion and cell killing assays. Simply load the reagents and samples, put the cartridge in Xdrop, and run the program.

  • Run 8 samples simultaneously or individually
  • Produce up to 500,000 droplets per lane in 8 minutes
  • Incubate droplets with cells in a CO2 incubator
  • Recover selected cells for expansion
Screenshot 2022 05 27 at 10.30.24

Natural killer cells and lymphoblasts co-encapsulated in DE50 droplets, which have a 105 pl volume and 58 µm inner diameter.

Xdrop DE20 Cartridge for Website

Xdrop® DE20 Cartridge

For encapsulating microbial cells, organelles, or long fragments of DNA in double-emulsion droplets

Produce highly stable, ~1.5-picoliter, double-emulsion droplets to encapsulate millions microbial cells, organelles, or DNA with the self-contained Xdrop DE20 Cartridge. Each droplet acts as a compartment for single-cell resolution assays or targeted enrichment of DNA. Simply load the reagents and samples, put the cartridge in Xdrop or Xdrop Sort, and run the program.

  • Run 8 samples simultaneously or individually
  • Produce up to 10 million droplets per lane in 45 minutes
  • Incubate droplets with cells in a CO2 incubator
  • Recover selected cells for expansion
  • Ensure a contamination-free workflow

Xdrop® DE20 Sort Cartridge

For sorting double-emulsion droplets containing DNA or small cells

Sort double-emulsion droplets containing fluorescing DNA or small cells with the new Xdrop DE20 Sort Cartridge. Designed for use with our new instrument, Xdrop Sort, this user-friendly system requires no expertise with fluorescence-based sorters. Simply load the reagents and samples, put the cartridge in Xdrop Sort, and run the sorting program.

  • Run up to 8 samples simultaneously 
  • Sort billions of droplets per day
  • Ensure a contamination-free workflow

Xdrop DE20 Sort Cartridge

Xdrop SE85 Cartridge for website

Xdrop® SE85 Cartridge

For encapsulating DNA, RNA, organelles, or small cells in single-emulsion droplets

Produce single-emulsion droplets to encapsulate millions of molecules, organelles, or cells with the self-contained Xdrop SE85 Cartridge. Each droplet acts as a compartment for single-molecule or single-cell resolution assays. Simply load the reagents and samples, put the cartridge in Xdrop or Xdrop Sort, and run the program.

  • Run 8 samples simultaneously or individually
  • Produce over 50,000 droplets per lane in 40 seconds
  • Ensure a contamination-free workflow

Xdrop Sort with people

How is Xdrop Sort different from Xdrop?

Both instruments run targeted DNA enrichment workflows  to prepare genomic material from large libraries for screening. They can both also encapsulate living cells for a range of cell-based applications.

Xdrop Sort uniquely enables DNA or cell encapsulation and high-throughput sorting. This enables the screening of large, complex gene libraries with unprecedented speed. 

Xdrop® Sort

Currently available in our early access program: please enquire

This new member of the Xdrop family can both generate and sort double-emulsion droplets in a user-friendly workflow that does not require any experience with fluorescence-based cell sorters.

The workflow starts with capture of 100 kb DNA fragments, small single cells, or other biological material in highly stable droplets for assays like PCR and enzyme activity assessment. Then, based on content fluorescence, droplets are sorted to obtain only the content of interest for high-resolution downstream analyses such as:

  • Verifying CRISPR edits without PCR bias that hides unintended rearrangements
  • Identifying viral and transgene integration sites
  • Assessing enzyme activity at the single-cell level
  • Performing high-throughput GFP screening

Specifications

Width: 30.5 cm   12 inches
Height: 36.4 cm   14.3 inches
Length: 65.4 cm   25.7 inches
Weight: 23.5 kg   51.8 lbs
Voltage requirements:  110-240 V

Bioinformatics tools

Calculator

Samplix provides upstream bioinformatics tools for primer design, determining input amounts and predicting enrichment. We're soon launching downstream tools for tasks like basecalling of Oxford Nanopore reads and generation of enrichment mapping reports. These tools are provided free of charge to Samplix customers.

Calculate the optimal DNA input range and predict and evaluate the target enrichment using our dedicated calculator set.

Primer design tool

Use the primer design tool to design all the primer pairs for target enrichment using Xdrop: the required number of droplet PCR primers for the detection sequence and qPCR primer pairs for the validation sequence.

Perform downstream analyses on Oxford Nanopore Technologies and Illumina data

Use these tools to handle Oxford Nanopore Technologies and Illumina data. They perform tasks like basecalling, reference downloads, and generating reports.

Bioinformatics lab
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