Xtalks & Samplix webinar

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Droplet-based Whole Genome Amplification: Successfully Sequencing Minute Amounts of Mycobacterium tuberculosis DNA

Discover this excellent webinar presented by Dr. Anzaan Dippenaar from University of Antwerp

By watching this webinar you'll gain insights into:

  • Efficient amplification for WGS: dMDA gives efficient amplification of minute amounts of Mycobacterium tuberculosis DNA, thus enabling precise WGS
  • Precision insights for tuberculosis genomics: dMDA along with Illumina WGS for samples with ≥5-pg DNA provides detailed insights into drug resistance, phylogeny and transmission dynamics in tuberculosis, offering a valuable tool for advancing genomics in infectious diseases
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Enzyme Engineering Advancements with Xdrop and Flow Cytometry

Discover how Xdrop and flow cytometry enhance the efficiency of enzyme engineering by boosting throughput for fluorescence-based screening.

In this webinar, Volkan Besirlioglu (Samplix) talked about: 

  • The limitation of conventional enzyme screening and the need for ultra high-throughput screening in enzyme engineering
  • Advancements in enzyme variant selection and analysis with Samplix’s Xdrop technology
  • Improving enzyme engineering efficiency by increasing the throughput of fluorescence-based screening with Xdrop

SelectScience webinar featuring Samplix and Agilent

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Double emulsion flow cytometry: Opening new frontiers in cell therapy analysis with Xdrop and NovoCyte

In this webinar, Dr. Garret Guenther (Agilent) demonstrates how the power of Agilent’s NovoCyte Quanteon flow cytometry coupled with Samplix’s Xdrop double-emulsion droplets helps characterize cytokine secretion and cell–cell interaction. Dr. Bárbara Schlicht (Samplix) presents our latest data on single-cell functional screening assays.

Talk from the Natural Killer Cell Symposium 2023

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Achieving single-cell resolution for cytokine secretion and cell-killing assays with Xdrop

In this talk, Dr. Peter Mouritzen discusses how double-emulsion droplet-based technology is changing the approach to cytokine secretion and cell-killing assays by changing the focus from bulk to true single-cell views. He shares the results of assays done in Xdrop double-emulsion droplets as well as describing the technology.

Applied Synthetic Biology in Europe VI (ASBE VI) webinar

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From bulk to single-cell formats: Xdrop changes how we work with yeast

In this video, Jinglin Wang of the SBTY Group at DTU discusses how using mono disperse double-emulsion (DE) droplets generated with Xdrop allow the encapsulation of living yeast cells, supporting single-cell format analyses of yeast as well as sorting to obtain the cells with the desired activity or properties.

Oxford Global Conferences webinar

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Building on a New Approach to Validate Gene Editing and Localize CAR Cassettes in T Cells

Safety and efficacy are key considerations in engineered cell therapies. However, conventional PCR screening for editing outcomes can overlook unintended cassette insertions. Here, Dr. Peter Mouritzen discusses an Xdrop workflow that is proven to overcome this limitation to identify the precise localization of the CAR insertion. The workflow also has applications in validating gene editing.

European Society of Human Genetics 2022

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Target: Rare Disease Insights

At ESHG 2022 in Vienna, Samplix hosted a special event where three experts talked about our novel technology that supports important research into the genetics of rare diseases.

Dr. Peter Mouritzen (Samplix): Xdrop: Targeting Rare Disease Genomics

Professor Alexander Hoischen (Radboud University Medical Center): Indirect targeted accurate long-read sequencing for rare diseases

Professor Sarah Cumming (Institute of Molecular Cell & Systems Biology, University of Glasgow): Xdrop technology can capture genomic DNA fragments that carry the myotonic dystrophy type 1 mutation

SLAS Europe 2022

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Achieving single-cell resolution for cytokine and cell-killing assays with Xdrop

Our CTO and co-founder, Dr. Marie Mikkelsen, describes how the microfluidics technology in Xdrop helps achieve single-cell resolution in important assays for immunotherapy research, such as cytokine secretion and killer cell activity assays. She also talks about why this single-cell resolution is so important.

Festival of Genomics & Biodata 2022

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Mapping and characterization of mouse transgenes using Xdrop technology

Hear how Dr. Lydia Teboul (Head of Molecular and Cellular Biology at the Mary Lyon Centre, MRC Harwell) and her team use the Xdrop target enrichment workflow to map and characterize mouse transgenes.

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How microfluidics is revolutionizing cell and DNA preparation

Our co-founder and CTO, Dr. Marie Just Mikkelsen, gives a talk on the role of microfluidics technology in boosting the throughput and resolution of gene and cell screening. Instruments such as Xdrop and Xdrop Sort are revolutionizing DNA and cell preparation for downstream analyses by enabling processes such as targeted DNA enrichment for sequencing, single-cell phenotyping, and single-cell assessments of enzyme activity.

NextGenOmics UK 2021

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Functional validation of multiomics data – CRISPR perks and nuisances

Dr. Claudia Kutter, Karolinska institute, talks about how her lab uses CRISPR editing to elucidate the role of non-coding RNAs in transcriptional regulation. She tells about how the lab optimized CRISPR editing to modify cell lines but discovered unintended genomic rearrangements that were otherwise unidentified by traditional methods. Here she shows how they were able to identify unintended edits by using Xdrop targeted enrichment combined with long-read sequencing

ESHG 2021 – Samplix Corporate Session

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Long-read whole genome analysis of single human cells

Talk by DR. ADAM AMEUR, Uppsala University and Science for Life Laboratory (SciLifeLab), at ESHG 2021 Virtual.

Leveraging the unbiased droplet multiple displacement amplification (dMDA) step of Xdrop®, Dr. Ameur prepared long fragments of gDNA from a single cell for HiFi PacBio sequencing. 2.5 million reads per cell and up to 40% genome coverage revealed comparable numbers of SNV as seen with short-read sequencing, but four times as many structural variants. The method presented goes far beyond limits of current long-read sequencing protocols, which normally require at least 5 ng of input DNA.

Samplix Webinar – Pitfalls and best practices for CRISPR/Cas9-based gene editing


Another two-part webinar featuring a collaboration with Bioneer. Benjamin gives specific recommendations for planning a successful gene editing strategy in "Ten things you should think of when designing a good gene editing strategy". Then, Peter provides advice for subsequent validation of your edits in "How to ensure your gene editing validation accounts for larger unintended rearrangements."

Samplix Webinar – Certainty After CRISPR

PAUL ERIC BENNETT, COBO Technologies, and KEYI GENG, Karolinska Institute.

A two-part webinar discussing unanticipated alterations happening after gene editing and ways to detect and characterize expected and unexpected outcomes. In "Fast and sensitive detection of indels induced by precise gene targeting", Paul surveys the pros and cons of current InDel detection methods and how to choose the one that is fit for purpose. Keyi describes her detective work in uncovering and fully characterizing unexpected outcomes of a gene editing strategy in "Where did the target region go? Unravelling odd CRISPR/Cas9-induced genomic alterations in human cells."

Genome Editing Oxford 2021 Virtual

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Validation of Genome Editing Through Indirect Sequence Capture and Long-Read Sequencing

Talk by PETER MOURITZEN, Vice President Application and Market Development, Samplix, at Genome Editing 2021 Virtual.

• Methods commonly used to validate genome editing may fail to detect unintended modifications occuring during editing with considerable ramifications for results.
• By enriching long DNA fragments over and around the region of interest, the Xdrop® indirect sequence capture provided by Samplix® offers a novel high-resolution alternative validation.
• Show case: Xdrop-based validation reveals an unwanted alteration in an engineered human cell line.

Festival of Genomics 2021

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Identification of functional proteins densely co-encoded in the human genome

Talk by ALEX KHITUN, Postdoctoral Researcher at Harvard Medical School

  •  Description of small open reading frame (ORF)-encoded proteins
  •  Discovery via buttom-up proteomics of small ORF-encoded proteins overlapping RPL36
  •  Assessment of structure and function of these densely encoded proteins
  •  Validation of knockout cell lines using Xdrop®
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CRISPR validation and resolving complex genomics landscapes with Xdrop

Talk by PETER MOURITZEN, VP Application & Market Development at Samplix

  •  Indirect Sequence Capture of any genomic region: the Xdrop® microfluidics system
  • Validation of CRISPR edited cell lines detecting unintended kb-long insertions
  • Identification of integration sites and structural variation in transgenic mice
  • Reconstruction of complex genomic regions: CYP2D6 gene without confounding effects of pseudogenes
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Genomically humanized mouse models of neurodegenerative disease

Talk by TOM CUNNINGHAM, Senior Investigator Scientist at MRC Harwell

  • Engineering and studying a new generation of knock-in mouse models of motor neuron disease and frontal temporal dementia
  • Genomic humanisation refers to replacement of entire mouse genes with the human orthologous sequence
  • Allele QC is critical in order to accurately study the consequences of pathogenic mutations

NextGen Omics Nov 2020 Virtual

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Tackling Disease Related Repeat- Expansion Analysis By Xdrop® Indirect Sequence Capture

Talk by MARZIA ROSSATO, Researcher in Genetics, University of Verona, at the NextGen Omics conference 2020.

• The analysis of long DNA fragments provides consistent benefits in the characterization of clinically-relevant regions with challenging features, such as tandem repeats, structural variants, and high CG-content
• The Xdrop indirect sequence capture provided by Samplix® represents a flexible approach to enrich for long DNA fragments of interest and sequence them using either short or long reads
• Show case: Xdrop can be efficiently applied for the analysis of SNV, tandem repeat length and interruption in genes underlying neurological disorders

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Xdrop – Validation Of Correct CRISPR Gene Editing

Talk by PETER MOURITZEN, Vice President Application and Market Development, Samplix, at NextGen Omics conference 2020

The Xdrop concept of Indirect Sequence Capture allows on-target analysis of 40 kb or more of the genomic region surrounding gene editing sites
• Case study: Detection of unintended on-target editing in a set of IPS cell lines
• Additional strategies for targeted enrichment to analyse for CRISPR on- and off- target events, CRISPR knock-in and other transgene integration patterns

Samplix Webinar June 2020

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Samplix Webinar: Capture the dark genome: From repeat-expansions to CRISPR unintended mutations

PART 1 (01:30): Tackling Disease-Related Repeat-Expansion Analysis - Dr. Marzia Rossato, Researcher, Functional Genomic Lab, University of Verona, Italy

PART 2 (20:44): Validation of CRISPR in a 100 kb Region Surrounding the Editing Site - Dr. Peter Mouritzen, Application Development Services, Samplix, Denmark

PART 3 (37:50): The Launch of a Grant Program and Q&A