Accessible to every lab, Xdrop enables screening of millions of antibody-secreting cells and isolation of high-value variants.
Identify and isolate rare antibody-secreting cells from your primary sample. Encapsulate millions of single cells in double emulsion droplets for high-throughput screening with flow cytometry or cell sorting. Effortlessly recover living cells producing the desired antibodies for downstream analysis and expansion.
Plasma cells from immunized mice are screened for antigen-specific antibody secretion in a one-day workflow. V(D)J sequencing yield 2,510 and 1,153 sequences from ~1 mllion screened plasma cells per mouse. This large-scale data generation provides a strong foundation for applying AI in antibody discovery.
Figure: Image of droplets containing the Xdrop ASC screening assay taken with Xcyto5, ChemoMetec. Cells secreting anti-hTNFα-antibodies clearly show an accumulated fluorescent signal on the TNFα-coated microspheres (A and B). Panels C and D show two droplets retaining a highly concentrated signal following sorting. Panels A and C were overlaid with brightfield images.
Transform discovery workflows. Effortlessly encapsulate single-cell libraries, enhancing the speed of your screening campaigns.
Explore immune cell function in single-cell format assays, including cytokine, granzyme B, and cell killing assays.
Advance genomics workflows with precise single DNA fragment encapsulation. Ideal for insert validation and targeted enrichments.
