How much DNA should I add to get desired target enrichment?
How much target enrichment can I expect from my sample?
What was my target enrichment in my experiment?
Use our primer design tool to desing all necessary primer pairs for target enrichment, providing the required Detection Sequence primers for droplet PCR and Validation Sequence primer pairs for qPCR verification.
For maximum control of your target sequence, you can choose to enter a FASTA sequence as the target for the primer design tool. This will ensure that the primers target the exact sequence of interest.
Use this option if your target sequence is not accurately described by the reference genome assemblies.
Find your target of interest by searching the Ensembl reference database. This will allow you to search using gene names, transcript names, and descriptions. This will also allow you to visualize gene splice variants.
Use this option if your target sequence is accurately described by the reference genome assemblies.
Explore our bioinformatics tools for streamlined handling of Oxford Nanopore Technologies and Illumina data. Discover detailed instructions on running these tools in the main manual.
Please ensure you are signed into a Github account to access the manual and download the required Docker image.
We provide tools for basecalling of Oxford Nanopore Technologies reads and for generating independent basecalling reports.
We provide tools for reference and annotation downloads, scaffold merging, and to generate enrichment mapping reports.
We provide tools for generating enrichment mapping reports and adding alignment tags.
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