Start with high molecular weight DNA and primers designed for droplet PCR
Partition and select long DNA fragments of interest with Indirect Sequence Capture
Separately amplify each selected long DNA fragment by droplet MDA
Use your 100–1000x enriched target in long- or short-read sequencing
Primers used for Target detection, not for Target amplification.
Use Xdrop to encapsulate DNA fragments into millions of droplets
A short (~150 bp) sequence that identifies the Target DNA is amplified
Target-positive droplets are stained green
A cell sorter is used to isolate green-stained positive droplets
Break solution is added to release Target native DNA from droplets
Xdrop generates thousands of droplets, each with single Target DNA + amplification reagents
Each molecule of Target DNA undergoes multiple displacement amplification in droplets
Break solution releases Samplicons (amplified Target DNA) into solution
Target is enriched 100-1000x and is ready for long- and short-read sequencing, qPCR etc.
Ready for applications....
Find the complete product portfolio including microfluidics cartridges and reagents for the Xdrop system.
Partner with the Samplix® Service Team to answer complex genomics questions with Xdrop