The Xdrop™ Technology

Enriched long DNA fragments from low sample input of largely unknown sequence. For everyone. Curious? Keep reading.

What makes Xdrop™ special?

LONG-RANGE GENOMIC INFORMATION
capture long fragments of DNA (>100 kb)

SMALL INPUT, LARGE OUTPUT
go from ng to µg amounts of DNA

LEARN MORE FROM EACH SEQUENCING RUN
with a straightforward enrichment method

WORK WITH UKNOWN SEQUENCES
you just need to know a short piece within or flanking the target

SINGLE-MOLECULE RESOLUTION
gain an unbiased view of your target’s variation

 

How it works - a quick overview

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Start with high molecular weight DNA and primers designed for droplet PCR

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Partition and select long DNA fragments of interest with Indirect Sequence Capture

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Separately amplify each selected long DNA fragment by droplet MDA

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Use your 100–1000x enriched target in long- or short-read sequencing

Here’s how it works in detail

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1

Mix High Molecular Weight (HMW) DNA, Primers and droplet PCR Reagents

Primers used for Target detection, not for Target amplification.

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Encapsulate DNA

Use Xdrop™ to encapsulate DNA fragments into millions of droplets


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Detection Sequence Amplification

A short (~150 bp) sequence that identifies the Target DNA is amplified


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Stain

Target-positive droplets are stained green


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Select Target-positive Droplets

A cell sorter is used to isolate green-stained positive droplets

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Release HMW Target DNA

Break solution is added to release Target native DNA from droplets


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Encapsulate Target DNA

Xdrop™ generates thousands of droplets, each with single Target DNA + amplification reagents


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Unbiased Samplicon Production

Each molecule of Target DNA undergoes multiple displacement amplification in droplets


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Release of ~ 1µg of Target DNA

Break solution releases Samplicons (amplified Target DNA) into solution

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Downstream Analysis

Target is enriched 100-1000x and is ready for long- and short-read sequencing, qPCR etc.

Ready for applications....

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Brochure workflow SAMPLIX A3

Products

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Find the complete product portfolio including
microfluidics cartridges and reagents for the Xdrop™ system.


Services

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Partner with the Samplix Service Team to answer
complex genomics questions with Xdrop™